Rat CTR CRE-Luc CHO
CBP71656
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| I. Background | |
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大鼠CTR（降钙素受体）由Calcr基因编码，属于B类G蛋白偶联受体家族。它在中枢神经系统（如伏隔核、孤束核等脑区）和外周组织（如肾脏、乳腺等）均有表达。大鼠CTR存在C1a和C1b两种异构体，C1a对鲑鱼降钙素亲和力高但对人/大鼠降钙素亲和力低；C1b因胞外域多了37个氨基酸的插入，对配体亲和力更低。此外，CTR与RAMP蛋白共表达时可形成功能性胰淀素受体，其也是破骨细胞的重要标志物。 |
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| II. Description | |
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Rat CTR CRE-Luc CHO 药靶模型很好的模拟了体内 Rat CTR 的信号转导过程。 |
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| III. Introduction | |
| Expressed gene: | Rat CTR |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K + 10%FBS + 5μg/ml Puromycin + 600μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional assay for Rat CTR |
| Transducer: | Gs |
IV. Representative Data Figure 1. Dose Response of Agonists in Rat CTR CRE-Luc CHO(C20) .  Figure 2. HTRF cAMP Assay with Rat CTR CRE-Luc CHO(C20) . |
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